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Stress responsive zinc-finger protein gene of Populus euphratica in tobacco enhances salt tolerance.

Identifieur interne : 003803 ( Main/Exploration ); précédent : 003802; suivant : 003804

Stress responsive zinc-finger protein gene of Populus euphratica in tobacco enhances salt tolerance.

Auteurs : Jun-Ying Wang [République populaire de Chine] ; Xin-Li Xia ; Jun-Ping Wang ; Wei-Lun Yin

Source :

RBID : pubmed:18666952

Descripteurs français

English descriptors

Abstract

The Populus euphratica stress responsive zinc-finger protein gene PSTZ, which encodes a protein including typical Cys(2)/His(2) zinc finger structure, was isolated by reverse transcription-polymerase chain reaction from P. euphratica. Northern hybridization revealed that its expression was induced under drought and salt stress conditions. To examine its function, cDNA of the PSTZ gene, driven by the cauliflower mosaic virus 35S promoter, was cloned into a plant expression vector pBin438 and introduced into tobacco plants. Transgenic tobacco showed an enhanced salt tolerance, suggesting that PSTZ may play a role in plant responsiveness to salt stress.

DOI: 10.1111/j.1744-7909.2007.00590.x
PubMed: 18666952


Affiliations:


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Le document en format XML

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<nlm:affiliation>Biotechnology Research Institute of Chinese Academy of Agricultural Sciences, Beijing 100081, China, and Beijing Forestry University, Beijing 100083, China.</nlm:affiliation>
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<term>Molecular Sequence Data (MeSH)</term>
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<term>Plant Proteins (physiology)</term>
<term>Plants, Genetically Modified (drug effects)</term>
<term>Plants, Genetically Modified (genetics)</term>
<term>Plants, Genetically Modified (growth & development)</term>
<term>Populus (genetics)</term>
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<term>Seedlings (drug effects)</term>
<term>Seedlings (genetics)</term>
<term>Seedlings (growth & development)</term>
<term>Sodium Chloride (pharmacology)</term>
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<term>Adaptation physiologique (physiologie)</term>
<term>Chlorure de sodium (pharmacologie)</term>
<term>Doigts de zinc (génétique)</term>
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<term>Plant (croissance et développement)</term>
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<term>Adaptation physiologique</term>
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<term>Régulation de l'expression des gènes végétaux</term>
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<term>Végétaux génétiquement modifiés</term>
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<div type="abstract" xml:lang="en">The Populus euphratica stress responsive zinc-finger protein gene PSTZ, which encodes a protein including typical Cys(2)/His(2) zinc finger structure, was isolated by reverse transcription-polymerase chain reaction from P. euphratica. Northern hybridization revealed that its expression was induced under drought and salt stress conditions. To examine its function, cDNA of the PSTZ gene, driven by the cauliflower mosaic virus 35S promoter, was cloned into a plant expression vector pBin438 and introduced into tobacco plants. Transgenic tobacco showed an enhanced salt tolerance, suggesting that PSTZ may play a role in plant responsiveness to salt stress.</div>
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